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Author:
Date:
2013-06-05
[Abstract] 2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012). Both dimensions consist of a conventional SDS-PAGE, except that the sample buffer for the first dimension lacks a reducing agent. Intermolecular disulfide bridges increase the apparent molecular weight of a protein in the first dimension, whereas intramolecular bridges decrease the apparent weight of the protein.
[摘要] 在该实施例中，使用蛋白质的2D对角线氧化还原SDS-PAGE用于检测分子内或分子间二硫键（Stroeher和Dietz，2008; Schwarz等人，2012） 。 两个维度由常规SDS-PAGE组成，除了第一维的样品缓冲液不含还原剂。 分子间二硫桥在第一维中增加蛋白质的表观分子量，而分子内桥降低蛋白质的表观重量。
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