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Author:
Date:
2013-09-05
[Abstract] Endo-β–mannanases in plant require post-translational modification, such as N-glycosylation and disulfide-linked dimerization, for their catalytic activity. Determination of the plant endo-β–mannanase activity needs to modify the assay conditions for optimizing their enzymatic reaction. Here, we describe a modified method for plant endo-β–mannanase assay. A high-salt buffer without thiol reductants is required for effective extraction of the enzyme. The enzyme is able to digest water-insoluble AZCL galactomannan to release water soluble dyed fragments, which is detected through measurement of ...
[摘要] 对于其催化活性，植物中的内切-β-甘露聚糖酶需要翻译后修饰，例如N-糖基化和二硫键连接的二聚化。 植物内切-β-甘露聚糖酶活性的测定需要改变测定条件以优化它们的酶反应。 在这里，我们描述了植物内切-β-甘露聚糖酶测定的修改方法。 为了有效地提取酶，需要没有硫醇还原剂的高盐缓冲液。 该酶能够消化水不溶性AZCL半乳甘露聚糖以释放水溶性染色片段，其通过测量在590nm波长处的吸光度来检测。 在590nm处的吸光度的增加与酶活性直接相关
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